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rabbit polyclonal antibody against rat inos  (Boster Bio)


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    Structured Review

    Boster Bio rabbit polyclonal antibody against rat inos
    Figure 5. Immunohistochemical staining of <t>iNOS</t> in rat retinas at 1 day after I/R. (A) sham group; (B) I/R group; (C) control group; (D) fasudil group. The rats in I/R group and control group exhibited a remarkable increase in iNOS reactivity (n=5).
    Rabbit Polyclonal Antibody Against Rat Inos, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+rat+inos/Anti-Phospho-NOS2+(Y151)+Antibody/pm21455561-59-1-8
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal antibody against rat inos - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats."

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.

    Journal: International journal of molecular medicine

    doi: 10.3892/ijmm.2011.659

    Figure 5. Immunohistochemical staining of iNOS in rat retinas at 1 day after I/R. (A) sham group; (B) I/R group; (C) control group; (D) fasudil group. The rats in I/R group and control group exhibited a remarkable increase in iNOS reactivity (n=5).
    Figure Legend Snippet: Figure 5. Immunohistochemical staining of iNOS in rat retinas at 1 day after I/R. (A) sham group; (B) I/R group; (C) control group; (D) fasudil group. The rats in I/R group and control group exhibited a remarkable increase in iNOS reactivity (n=5).

    Techniques Used: Immunohistochemical staining, Staining, Control

    Figure 6. iNOS mRNA and protein levels in rat retinas at 1 day after I/R were measured by quantitative real-time RT-PCR (A) and Western blot analysis (B), respectively. (A) Compared to the sham group, there was a marked increase in iNOS mRNA in the I/R group and the control group. Fasudil pre-treatment significantly attenuated the increase of iNOS mRNA in retinas of rats with I/R. (B) iNOS protein levels in the above-mentioned groups were consistent with the mRNA expression by Western blotting. Representative blots are shown, and the protein size is expressed in kDa. (n=5, *P<0.05 vs. the sham group, #P<0.01 vs. the control group).
    Figure Legend Snippet: Figure 6. iNOS mRNA and protein levels in rat retinas at 1 day after I/R were measured by quantitative real-time RT-PCR (A) and Western blot analysis (B), respectively. (A) Compared to the sham group, there was a marked increase in iNOS mRNA in the I/R group and the control group. Fasudil pre-treatment significantly attenuated the increase of iNOS mRNA in retinas of rats with I/R. (B) iNOS protein levels in the above-mentioned groups were consistent with the mRNA expression by Western blotting. Representative blots are shown, and the protein size is expressed in kDa. (n=5, *P<0.05 vs. the sham group, #P<0.01 vs. the control group).

    Techniques Used: Quantitative RT-PCR, Western Blot, Control, Expressing

    Related Articles

    Immunohistochemical staining:

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.
    Article Snippet: Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.. A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.

    Staining:

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.
    Article Snippet: Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.. A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.

    Control:

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.
    Article Snippet: Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.. A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.

    Quantitative RT-PCR:

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.
    Article Snippet: Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.. A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.

    Western Blot:

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.
    Article Snippet: Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.. A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.

    Expressing:

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.
    Article Snippet: Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.Following deparaffinization of the sections, immunohistochemical staining was performed with streptavidin-biotinylated peroxidase to detect the expression of iNOS, as described elsewhere.. A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.



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    Image Search Results


    Immunohistochemistry (200x) of inducible nitric oxide synthase (iNOS) of rat kidneys from (A) negative control group showing no staining (NS); (B) positive control group showing an obviously increased level of iNOS immunostaining in brown color; (C) Naringenin 100 mg /kg + cyclophosphamide group showing a marked decrease in iNOS positivity, and (D) the Naringenin 200 mg /kg + cyclophosphamide group showing no staining (NS).

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    Figure Lengend Snippet: Immunohistochemistry (200x) of inducible nitric oxide synthase (iNOS) of rat kidneys from (A) negative control group showing no staining (NS); (B) positive control group showing an obviously increased level of iNOS immunostaining in brown color; (C) Naringenin 100 mg /kg + cyclophosphamide group showing a marked decrease in iNOS positivity, and (D) the Naringenin 200 mg /kg + cyclophosphamide group showing no staining (NS).

    Article Snippet: Sections were pre-treated in buffer citrate (10 mM, pH 6.0) in a microwave and incubated with rabbit polyclonal antibodies against rat inducible nitric oxide synthase (iNOS) (Thermo Scientific, USA; 1:100), and kidney injury molecule-1 (KIM-1) (1:50; Thermo Scientific, USA).

    Techniques: Immunohistochemistry, Negative Control, Staining, Positive Control, Immunostaining

    Figure 5. Immunohistochemical staining of iNOS in rat retinas at 1 day after I/R. (A) sham group; (B) I/R group; (C) control group; (D) fasudil group. The rats in I/R group and control group exhibited a remarkable increase in iNOS reactivity (n=5).

    Journal: International journal of molecular medicine

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.

    doi: 10.3892/ijmm.2011.659

    Figure Lengend Snippet: Figure 5. Immunohistochemical staining of iNOS in rat retinas at 1 day after I/R. (A) sham group; (B) I/R group; (C) control group; (D) fasudil group. The rats in I/R group and control group exhibited a remarkable increase in iNOS reactivity (n=5).

    Article Snippet: A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.

    Techniques: Immunohistochemical staining, Staining, Control

    Figure 6. iNOS mRNA and protein levels in rat retinas at 1 day after I/R were measured by quantitative real-time RT-PCR (A) and Western blot analysis (B), respectively. (A) Compared to the sham group, there was a marked increase in iNOS mRNA in the I/R group and the control group. Fasudil pre-treatment significantly attenuated the increase of iNOS mRNA in retinas of rats with I/R. (B) iNOS protein levels in the above-mentioned groups were consistent with the mRNA expression by Western blotting. Representative blots are shown, and the protein size is expressed in kDa. (n=5, *P<0.05 vs. the sham group, #P<0.01 vs. the control group).

    Journal: International journal of molecular medicine

    Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.

    doi: 10.3892/ijmm.2011.659

    Figure Lengend Snippet: Figure 6. iNOS mRNA and protein levels in rat retinas at 1 day after I/R were measured by quantitative real-time RT-PCR (A) and Western blot analysis (B), respectively. (A) Compared to the sham group, there was a marked increase in iNOS mRNA in the I/R group and the control group. Fasudil pre-treatment significantly attenuated the increase of iNOS mRNA in retinas of rats with I/R. (B) iNOS protein levels in the above-mentioned groups were consistent with the mRNA expression by Western blotting. Representative blots are shown, and the protein size is expressed in kDa. (n=5, *P<0.05 vs. the sham group, #P<0.01 vs. the control group).

    Article Snippet: A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.

    Techniques: Quantitative RT-PCR, Western Blot, Control, Expressing